General Cell Based Assays
Meso Scale Discovery RPLEX Hu Antileukoproteina ASY
The R-PLEX Human Antileukoproteinase Assay from Meso Scale Discovery is an electrochemiluminescent immunoassay designed for the quantitative measurement of human antileukoproteinase (ALP) in serum plasma and cell culture supernatants The assay utilizes MSD s proprietary SULFO-TAG labels and multi-array plate technology to provide sensitive and rapid detection of human ALP This assay can be used to study ALP levels in disease research biomarker discovery and drug development With high sensitivity broad dynamic range and minimal matrix interference the R-PLEX ALP assay enables robust and reproducible quantification of this important serine protease inhibitor
Cayman Chemical 33-BCRP InhIbItr 1mg
A BCRP inhibitor; sensitizes mitoxantrone-resistant H460/MX20 cells to mitoxantrone-induced cell death alone and in combination with UV radiation; sensitizes KB-C2 cells overexpressing P-gp to colchicine-induced cell death; increases intracellular accumulation of mitoxantrone in H460/MX20 cells at 5 µM
Meso Scale Discovery RPLEX HUM p38 pT180/pY182 ASY
The R-Plex Human p38 pT180/pY182 Assay from Meso Scale Diagnostics is a multiplex immunoassay kit for measuring phosphorylated p38 MAP kinase at Thr180 and Tyr182 in human cell lysates It uses electrochemiluminescent detection for rapid sensitive and reproducible analysis of p38 phosphorylation The kit includes a 96-well plate with capture antibodies detection antibodies and calibrator solutions It monitors p38 activation due to cellular stresses and inflammatory signals with a 2-hour sample incubation for high-throughput profiling
Meso Scale Discovery R-PLEX Mouse/Rat TREM-1 ASY
The TREM-1 Human ProcartaPlex Simplex Kit is a multiplex immunoassay that measures TREM-1 in human samples such as serum plasma and cell culture supernatants This assay uses Luminex bead-based technology and provides reagents for the quantitative determination of human TREM-1 concentrations The kit contains a 96-well plate coated with analyte capture antibodies detection antibodies and standards to generate a standard curve to calculate analyte concentrations This assay allows for the simple and rapid profiling of human TREM-1 levels in approximately 4 hours
Revvity Health Sciences Inc HTRF Terbium cryptate Labeling Detection Kit
HTRF Terbium cryptate Labeling Detection Kit
Sigma Aldrich Fine Chemicals Biosciences Ras Assay Reagent Raf 1 RB
Product Source Human Ras Binding Domain (RBD residues 1-149) of Raf-1 expressed in E. coli.
Sigma Aldrich Fine Chemicals Biosciences Sulfate Assay Kit sufficie
Inorganic sulfate is one of the most abundant anions in mammalian plasma. Sulfate plays important physiological roles in activating and detoxifying xenobiotics steroids neurotransmitters and bile acids. Sulfate is needed for the biosynthesis of cerebroside sulfate glycosaminoglycans and heparin sulfate. Undersulfation of cartilage proteoglycans has been associated with human inherited osteochondro-dysplasia disorders. In mammals sulfate homeostasis is regulated by the kidney. The majority of filtered sulfate is absorbed in the proximal tubules and only 5-20% of the filtered load is excreted into the urine.
Sigma Aldrich Fine Chemicals Biosciences Proteasome 20S Activity As
Protein degradation occurs most commonly through two major mechanisms lysosomal degradation and proteasome mediated degradation. Lysosomal degradation occurs through proteolytic enzyme activity and is fairly non-specific. Proteasomal degradation is targeted for a specific protein through the ubiquitination of the target protein. The target protein is typically poly-ubiquitinated and activates the 26S proteasome toward proteolysis. The proteasome 26S is the most common form of the proteasome and is an ATP-dependent proteolytic complex which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps.The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis DNA repair endocytosis and cell cycle and has been implicated in disease states such as certain types of cancer diabetes mellitus and Alzheimer disease among others making it the target f
Eurofins Discoverx PathHunter IL10RA/10RB Dimerization Bioassay Kit, 10pl. (93-0985Y3-00136)
The PathHunter IL10RA/IL10RB Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-10) induced dimerization of IL10RA/IL10RB receptors. This product uses EFC technology. The cell line included overexpresses IL10RA target protein fused to PK alongside IL10RB target protein fused to EA. Upon ligand-induced dimerization of IL10RA/IL10RB, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress cINSRa Functional Assay, 2-Plate Kit. (93-1016E3CP16M)
The PathHunter eXpress cINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated cINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged cINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL7R/CRLF2 Dimerization Assay, 2pl Kit. (93-1019E13CP25M)
The PathHunter IL7R/CRLF2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TSLP) induced dimerization of IL7R/CRLF2 receptors. This product uses EFC technology. The cell line included overexpresses IL7R target protein fused to PK alongside CRLF2 target protein fused to EA. Upon ligand-induced dimerization of IL7R/CRLF2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress RXFP4 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0701E2CP0L)
The PathHunter RXFP4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of RXFP4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged RXFP4 and EA-tagged β-Arrestin-2. Activation of RXFP4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress FFAR3 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0136E2CP2M)
The cAMP Hunter FFAR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. FFAs) based activation of FFAR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FFAR3, a naturally Gi/Go coupled GPCR. Upon activation of FFAR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress ADRA2A CHO-K1 GPCR Assay, 10-Plate Kit. (95-0126E2CP2L)
The cAMP Hunter ADRA2A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRA2A, a naturally Gi/Go coupled GPCR. Upon activation of ADRA2A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.