General Cell Based Assays
Meso Scale Discovery U-PLEX Human CA1 ASY
The Meso Scale Discovery U-PLEX Human CA1 Assay is a multiplex immunoassay kit for measuring CA1 in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection offering high sensitivity a dynamic range of up to 5 logs and low sample volume needs The kit includes a 96-well plate with capture antibodies detection antibodies with electrochemiluminescent labels calibrators and diluent It quantifies CA1 levels with minimal hands-on time using an MSD instrument completing tests in 3 5 hours for efficient CA1 biomarker analysis
Eurofins Discoverx PathHunter eXpress F2RL1 (PAR2; GPR11) U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0235E3CP0L)
The PathHunter F2RL1 (PAR2; GPR11) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. proteases, SLIGRL) based activation of F2RL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2RL1 and EA-tagged β-Arrestin-2. Activation of F2RL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Ethos Biosciences Exocell DTNB Thiol Oxidative Stress Assay, Ethos
The Ethos Biosciences DTNB (Ellman’s) Thiol Oxidative Stress Assay Kit is designed to measure free thiol groups that arise due to oxidative stress, which is an underlying component of acute and chronic kidney disease. Our kit uses Ellman’s Reagent otherwise known as DTNB or 5-5′-dithiobis [2-nitrobenzoic acid] which reacts with the disulfide bond in a sample to produce 2-nitro-5-thiobenzoic acid. Under conditions of oxidative stress, free thiols decrease and disulfides increase. Determination of the free thiol concentration reflects the ability to detoxify lipid (and other) peroxides. Aliquots of standard or sample are reacted with rehydrated Ellman’s reagent (DTNB) in microtiter wells. Our DTNB (Ellman’s) Thiol Oxidative Stress Assay kit contains all of the components and reagents you need to conduct a 96 well plate chemical assay for thiol groups of any species in up to 40 duplicate urine or cell free liquid samples (plasma, serum, cell culture supernatant, etc…)
Eurofins Discoverx PathHunter VIPR2 Bioassay Kit, 2-Plate. (93-0317Y2-00087)
The PathHunter VIPR2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. VIP) based activation of VIPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged VIPR2 and EA-tagged β-Arrestin-2. Activation of VIPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx cAMP Hunter eXpress CNR1 (CB1) CHO-K1 GPCR Assay, 2pl Kit. (95-0071E2CP2M)
The cAMP Hunter CNR1 (CB1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of CNR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CNR1, a naturally Gi/Go coupled GPCR. Upon activation of CNR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress ADCYAP1R1 (PAC1) CHO-K1 GPCR Assay, 10pl Kit. (95-0064E2CP2L)
The cAMP Hunter ADCYAP1R1 (PAC1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PACAP) based activation of ADCYAP1R1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADCYAP1R1, a naturally Gs coupled GPCR. Upon activation of ADCYAP1R1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CXCR2 (IL8RB) CHO-K1 GPCR Assay, 2-Plate Kit. (95-0074E2CP2M)
The cAMP Hunter CXCR2 (IL8RB) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of CXCR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR2, a naturally Gi/Go coupled GPCR. Upon activation of CXCR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPRL1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0264E2CP0M)
The PathHunter OPRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orphanin FQ) based activation of OPRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRL1 and EA-tagged β-Arrestin-2. Activation of OPRL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GRPR U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0229E3CP5M)
The PathHunter GRPR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. gastrin-releasing peptide) based activation of GRPR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GRPR and EA-tagged β-Arrestin-2. Activation of GRPR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GLP1R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0300E2CP0L)
The PathHunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-2. Activation of GLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR9 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0296E2CP0M)
The PathHunter CCR9 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL25) based activation of CCR9 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR9 and EA-tagged β-Arrestin-2. Activation of CCR9 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter GLP1 (7-37) Bioassay Kit, 2-Plate. (93-0300Y2-00027)
The PathHunter GLP1 (7-37) Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses GLP1 (7-37) based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-2. Activation of GLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress SSTR1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0307E3CP5L)
The PathHunter SSTR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR1 and EA-tagged β-Arrestin-2. Activation of SSTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR109A CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0301E2CP2M)
The PathHunter GPR109A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. nicotinic acid) based activation of GPR109A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR109A and EA-tagged β-Arrestin-2. Activation of GPR109A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.